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Stereomicroscopy_XG

作者:XG   发布时间:2015-02-05 17:25:19   浏览次数:519

Instruction for AXIO Zoom. V16

1)       Register for using (Name, Date, Number of the fluorescent meter)

2)       Start in order: EMS3 -> CL6000 LED, both if necessary -> X-cite Lymen dynamics -> computer and software ZEN lite 2012

3)       SYCOP 3-control of objective: central button: up and down-Z axis; left and right-magnification; two black buttons aside for micro-adjustment.

4)       ZEN lite 2012: Camera: Gain-binning 1X1, Color Mode B/W for fluorescent images

5)       Microscope components (very important to set parameters when you need to add the scare bar!!):

Objective 1.5X or 1.0 X;

Zoom=magnification (read number from SYCOP3)/objective;

Camera Adapter 0.63X

FIRST: ZEN: Home-set up-components ApoZ 1.5X or Plan NeoFluarZ 1.0X

SECOND: MTB: Newconfiguration-EMS3/syCop3-fix objective ApoZ 1.5X or Plan NeoFluarZ 1.0X

6)       You may need to regulate fluorescent intensity from the X-cite Lymen dynamics, aperture diaphragm from AXIO Zoom. V16, and the bottom light control at the bottom of the AXIO Zoom. V16.

7)       Processing-parameters click Burn-in Graphics, could save all your modification of your image.

8)       Concerning Z-stack: process your image with the following steps: sharpen-extended depth of focus-Method-wavelets.

9)       Save data: (save as *.czi or export as *.TIFF) in to D:creat your new folder.

10)   In case you lost your data, it could be find back from D:Auto save

11)   Switch off in order: software ZEN lite 2012 and computer -> CL6000 LED, -> X-cite Lymen dynamics-> EMS3.

12)   Put back all the cover protections for AXIO Zoom. V16 and computer.

 

Never touch the red “stop” button unless you want to stop the device when it is falling down by accident.

Any questions contact to Dr. Xin GUAN please, room 516, Tel: 68251047, E-mail: xinguan.cnde@googlemail.com

 

30.01.2015 Dr. Xin GUAN






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